medRxiv [Preprint]. 2025 Dec 6:2025.11.30.25341323. doi: 10.64898/2025.11.30.25341323.

ABSTRACT

The test-negative design (TND) has become a widely used observational study design for evaluating vaccine effectiveness (VE), especially during the COVID-19 pandemic. Traditionally, TND has often been viewed as a variant of the case-control study, with its analysis largely limited to logistic regression models. In this paper, we first establish that TND can be framed as a special case of a cohort study, thereby opening the door to a wider range of analytical approaches. We then introduce the Prentice, Williams, and Peterson gap-time (PWP-GT) frailty model as a novel method for analyzing TND data, accounting for recurrent infections and time-dependent vaccination status. Through extensive simulation studies, we demonstrate that the proposed model outperforms conventional models commonly applied in TND-based VE studies. Finally, we apply our method to data from the National COVID Cohort Collaborative (N3C), estimating the effectiveness of full and booster doses of Pfizer's COVID-19 vaccines against both initial infection and reinfection during the Omicron variant circulation period in a real-world setting.

PMID:41409669 | PMC:PMC12706598 | DOI:10.64898/2025.11.30.25341323